| 质粒名称: | pMK297 (DHC1-mAID Hygro) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 140542 |
| 存储实验室: | Masato Kanemaki |
|
载体骨架基本信息 |
|
| 载体名称: | pCR2.1 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达,CRISPR |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | DHC1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMK297 (DHC1-mAID Hygro) was a gift from Masato Kanemaki (Addgene plasmid # 140542 ; http://n2t.net/addgene:140542 ; RRID:Addgene_140542) |
| 参考文献部分: | The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y, Hayashi KI, Kanemaki MT.. Nat Commun (2020) 11(1):5701 10.1038/s41467-020-19532-z |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pMK297 (DHC1-mAID Hygro) 9446 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION DHC1 tagging with mAID.
ACCESSION .
VERSION .
KEYWORDS pMK297 (DHC1-mAID Hygro)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9446)
AUTHORS Yesbolatova A, Saito Y, Kitamoto N, Makino-Itou H, Ajima R, Nakano
R, Nakaoka H, Fukui K, Gamo K, Tominari Y, Takeuchi H, Saga Y,
Hayashi KI, Kanemaki MT.
TITLE The auxin-inducible degron 2 technology provides sharp degradation
control in yeast, mammalian cells, and mice.
JOURNAL Nat Commun (2020) 11(1):5701
REFERENCE 2 (bases 1 to 9446)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9446
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 1314..1517
/codon_start=1
/product="minimal degron from the auxin-responsive IAA17
protein of Arabidopsis thaliana (Kubota et al., 2013)"
/label=mini-AID
/note="acts as an auxin-inducible degron (AID) in the
presence of TIR1"
/translation="KEKSACPKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEA
AAFVKVSMDGAPYLRKIDLRMYK"
CDS 1524..2243
/codon_start=1
/product="bright green-yellow fluorescent protein derived
from mClover2, with improved photostability (Bajar et al.,
2003)"
/label=mClover3
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
EFVTAAGITHGMDELYK"
primer_bind complement(1569..1590)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind 2177..2198
/label=EGFP-C
/note="EGFP, forward primer"
polyA_signal 2361..2482
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2398..2417)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2452..2471
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
protein_bind complement(2513..2546)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter 2607..3106
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2642..2665)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 3039..3058
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 3167..4186
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
protein_bind complement(4749..4782)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind complement(5879..5898)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(5880..5898)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(5905..5922)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(5905..5921)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5914..5936)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6062..6490
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6149..6168)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6359..6380
/label=F1ori-F
/note="F1 origin, forward primer"
CDS 6834..7628
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6888..6907)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7498..7517
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
CDS 7646..8506
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7864..7883)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8677..9265
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 9166..9185
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9419..9436
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
241 gtaccttcag cgagaggatc aaacagctgc agaacatctc actggcagct gcatctggtg
301 gcgccaagga gctaaaggtg aaggcgctcc tgacgagtct cgggtggtca gcagctgtcc
361 tgggctgggg tgggagtggc tctggggaaa aacacagggc ccaggtctga cctgagctcc
421 ttcccctggg ggctgctgct ttccacagaa catccacgtg tgcctgggtg gcctgttcgt
481 gcctgaggcg tacatcactg ccaccaggca gtatgtggcc caggccaaca gctggtccct
541 ggaggagctc tgcctggaag tcaacgtcac cacctcacag ggcgccaccc ttgacgcttg
601 cagcttcgga gtcacgggtg agtggagtct cacagaaaat actggctctt tgcaggtgac
661 ctcggtggcc tgagaccatt gttcccagat acatgcactt agggtgaccg gctggcagtt
721 gggtggagcc tctgggcgcc ctgtgactgg ggtttgtgta gctgttgtgt tcacctcagc
781 ctgggttttg gcttccgcct cacaggtttg aaacttcaag gggccacgtg caacaacaac
841 aagctgtcac tgtccaatgc catctcaacc gcccttcccc tgacgcagct gcgctgggtc
901 aagcagacaa acaccgagaa gaaggccagt gtggtaagga ggcactgcct ttcccaggca
961 ttctgcaggg acccctgcgg taacaagggc agaggcggct cctcttctat gcctgggttc
1021 cacttggaac gggaaatgag gttcacagag gaaatccatt tcgcacctgt ccaaacctct
1081 ccttgccggg ccccatcagc tgtcccgggc agtcttccag ttttcttact tttcccttaa
1141 gccaccagta aacccctctg cttctgcagg taaccttacc tgtctacctg aacttcaccc
1201 gtgcagacct catcttcacc gtggacttcg aaattgctac aaaggaggac cctcgctcgt
1261 tctacgaaag aggtgtcgca gtcttgtgca cagagggatc cggtgcaggc gccaaggaga
1321 agagtgcttg tcctaaagat ccagccaaac ctccggccaa ggcacaagtt gtgggatggc
1381 caccggtgag atcataccgg aagaacgtga tggtttcctg ccaaaaatca agcggtggcc
1441 cggaggcggc ggcgttcgtg aaggtatcaa tggacggagc accgtacttg aggaaaatcg
1501 atttgaggat gtataaagct agcatggtga gcaagggcga ggagctgttc accggggtgg
1561 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtccgcggcg
1621 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
1681 agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcgtg gcctgcttca
1741 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
1801 acgtccagga gcgcaccatc tctttcaagg acgacggtac ctacaagacc cgcgccgagg
1861 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
1921 aggacggcaa catcctgggg cacaagctgg agtacaactt caacagccac aacgtctata
1981 tcacggccga caagcagaag aacggcatca aggctaactt caagatccgc cacaacgttg
2041 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc
2101 ccgtgctgct gcccgacaac cactacctga gccatcagtc caagctgagc aaagacccca
2161 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg attacacatg
2221 gcatggacga gctgtacaag taatctagat aactgatcat aatcagccat accacatttg
2281 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
2341 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
2401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
2461 ccaaactcat caatgtatct taacgcgtcg atcatattca ataaccctta atataacttc
2521 gtataatgta tgctatacga agttattagg tctgaagagg agtttacgtc cagccaagct
2581 taggatctcg acctcgaaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg
2641 agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg
2701 cacacattcc acatccaccg gtaggcgcca accgactccg ttctttggtg gccccttcgc
2761 gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg
2821 caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc
2881 tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc
2941 tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag
3001 gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg
3061 cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct gcatccatct
3121 agatctcgag cagctgaagc ttaccgctag catggataga tccggaaagc ctgaactcac
3181 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
3241 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
3301 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
3361 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct
3421 gacctattgc atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga
3481 actgcccgct gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct
3541 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
3601 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
3661 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
3721 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
3781 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
3841 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
3901 ctacttcgag cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct
3961 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
4021 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
4081 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
4141 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag gaataggcta gccgcccgcc
4201 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcga tgatatcaga
4261 tccccgggat gcagaaattg atgatctatt aaacaataaa gatgtccact aaaatggaag
4321 tttttcctgt catactttgt taagaagggt gagaacagag tacctacatt ttgaatggaa
4381 ggattggagc tacgggggtg ggggtggggt gggattagat aaatgcctgc tctttactga
4441 aggctcttta ctattgcttt atgataatgt ttcatagttg gatatcataa tttaaacaag
4501 caaaaccaaa ttaagggcca gctcattcct cccactcatg atctatagat ctatagatct
4561 ctcgtgggat cattgttttt ctcttgattc ccactttgtg gttctaagta ctgtggtttc
4621 caaatgtgtc agtttcatag cctgaagaac gagatcagca gcctctgttc cacatacact
4681 tcattctcag tattgttttg ccaagttcta attccatcag aagctggtcg agatccggaa
4741 cccttaatat aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgaagaggt
4801 tcactaggat ccacttttct agctgcccct ttctgtaata gtgaaagttg gtatttaaca
4861 tttattcatt tttaaaatat ttggaaggtc tgagcttgtg aaaagaaagt ggttggtctg
4921 aggttggagg aagctgaatg gaatctgacg gttgggagtg gtggaaattg gaaggatacc
4981 aggaggtatt tgggaaggcc aatggcgtgg ctcctttgag gaaataaaac actaagcatg
5041 agccggctcc gcctcttctg tctccgcttt catcccaggg cacagagcct tgccttccat
5101 gctgcccagg gagggcagcc cacggcagcc atgcccctcc ccacctcgct ttcatcatga
5161 gctcgctccc gagcggccac agcactcatg aatgaagacc ttggggccct tcacagacac
5221 agatgcagcc agctgtggct ctgaaggccc tggggcccgg gcaccatggt tcacaccttt
5281 aatcgcagca ctttgggagt ctgggagtta aagaccagcc tcggcaacat agtgagaccc
5341 cgtctctaca ggaaattaaa tcaggtgtgg tggtgcatgc ctgtagtccc agctacttga
5401 gggctgaggt gggaggatca cccaagccca agaggtcgag gctgcagtga gctgtgatct
5461 caccactgca ctccagcctg ggtgacagag caagaccctg tctcaaaaaa aaaaagctgg
5521 gcgtggtggc tcatgcctgt aatcccagca ctttgggagg ccgaggcggg cggatcacca
5581 aattagccgg gcatggtggc acatgcctgt aatcccagct actcgggagg ctgaggcagg
5641 agaattgctt gaacctggga ggcggaggtt gcagtgagct gaaaaaaaaa aaggcagccc
5701 ccagccgctt gtgttcttga ccagggcccc aggacttggc tcctccagac aagggagttt
5761 tgtgctgtag atgagggagt tgcccatcgc cgccctagca agtccattcc cacacgacct
5821 ttccagtggt gatgatgaca gtggcccact cgagcatgca tctagagggc ccaattcgcc
5881 ctatagtgag tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa
5941 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
6001 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
6061 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
6121 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
6181 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
6241 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
6301 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
6361 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
6421 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
6481 aacgcgaatt ttaacaaaat tcagggcgca agggctgcta aaggaagcgg aacacgtaga
6541 aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga
6601 caagggaaaa cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggcgat
6661 agctagactg ggcggtttta tggacagcaa gcgaaccgga attgccagct ggggcgccct
6721 ctggtaaggt tgggaagccc tgcaaagtaa actggatggc tttcttgccg ccaaggatct
6781 gatggcgcag gggatcaaga tctgatcaag agacaggatg aggatcgttt cgcatgattg
6841 aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg
6901 actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg
6961 ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg
7021 aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg
7081 ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc
7141 tgtcatccca ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc
7201 tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc
7261 gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc
7321 aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg
7381 atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct
7441 tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt
7501 tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc
7561 tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt
7621 tcttctgaat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
7681 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
7741 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
7801 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
7861 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
7921 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
7981 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
8041 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
8101 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
8161 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
8221 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
8281 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
8341 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
8401 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
8461 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
8521 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
8581 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
8641 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
8701 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
8761 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
8821 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
8881 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
8941 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
9001 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
9061 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
9121 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
9181 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
9241 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
9301 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
9361 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
9421 cgagtcagtg agcgaggaag cggaag
//
